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Live Cell Imaging

Incucyte display

All of the Facility's confocal and wide-field systems have some kind of environmental control; the systems most frequently used for live cell imaging are shown below. Our dedicated live cell imaging platform is a Sartorius Incucyte, which is a wide-field fluorescence microscope specifically designed to fit inside a standard tissue culture incubator.

Our systems:

Sartorius Incucyte SX5

A wide-field fluorescence microscope in an incubator

4x, 10x, 20x objectives

Green/Red and Green/Orange/Near-infrared optical modules

Ideal for longer term live cell imaging experiments running over days or weeks that requiring tightly regulated environmental conditions

Up to 6 separate vessels (multi-well plates, T75s etc), running different acquisition protocols for each vessel.

Common applications include monitoring cell growth and proliferation, expression of reporter genes, spheroid/organoid development, wound healing.

Incucyte
Olympus SpinSR

Olympus SpinSR Spinning Disk Confocal

Pecon full blackout enclosure

Temperature control up to 30掳C above ambient

Micro-chamber for gas and humidity regulation

CO2 control from 0.0 Vol-% up to 20.0 Vol-%

O2 control from 0.0 Vol-% up to 21.0 Vol-%

Nikon Ti-2 wide-field

Full enclosure black panel environmental control

Temperature range from 3掳C above ambient to 45掳C

Temperature measured in the chamber or at the sample

0.1掳C Temperature accuracy at sample

Micro chamber for humidity and CO2 

Ti2 oko